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On the Cover: MBP-specific transduced CD4⁺ T cells traffic to the CNS. Splenocytes from MBP TCR-Tg mice were transduced with the GFP-luciferase retroviral vector. CD4⁺ cells were isolated by MACS and analyzed by FACS for GFP expression. Cell suspensions with 1 X 10⁶ GFP⁺ cells were transferred i.v. to PL/J recipients. Recipients were anesthetized, and luciferin substrate was administered. Pseudocolor images range from least (blue) to most (red) luciferase photon intensity, and organs demostrating luciferase-positive cells are labeled as follows: LG, lung; CLN, cervical lymph node; ALN, axillary lymph node; BLN, branchial lymph node; ILN, inguinal lymph node; SPL, spleen; MLN, mesenteric lymph node; PLN, periaortic lymph node; IMM, sites of MBP/CFA immunization; and SC, spinal cord. A, A total of 1 X 10⁶ CD4⁺ cells transduced to express GFP- luciferase were transferred to naive or MBP-immunized mice 11 days after EAE induction but before EAE clinical signs. Data are representative of three experiments (n = 2-3/group). B, A total of 1 X 10⁶ CD4⁺ cells transduced to express GFP-luciferase were transferred to naive (n = 2) or MBP-immunized (n = 6) mice during EAE clinical signs (26 days after EAE induction, clinical score = 2). (Figure 4 from Costa, G. L., M. R. Sandora, A. Nakajima, E. V. Nguyen, C. Taylor-Edwards, A. J. Slavin, C. H. Contag, C. G. Fathman, and J. M. Benson. Adoptive immunotherapy of experimental autoimmune encephalomyelitis via T cell delivery of the IL-12 p40 subunit. J. Immunol. 167:2379.)

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