The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kuijpers, K. C.
Right arrow Articles by Miedema, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuijpers, K. C.
Right arrow Articles by Miedema, F.

The Journal of Immunology, Vol 144, Issue 9 3281-3287, Copyright © 1990 by American Association of Immunologists

ARTICLES

Analysis of the role of leukocyte function-associated antigen-1 in activation of human influenza virus-specific T cell clones

KC Kuijpers, TW Kuijpers, WP Zeijlemaker, CJ Lucas, RA van Lier and F Miedema
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands.

The role of leukocyte function-associated Ag-1 (LFA-1) in intercellular adhesion is well documented. Previously, we demonstrated that the LFA-1 molecule (CD11a/CD18) can also regulate the induction of proliferation of peripheral blood T cells. In these studies, we observed opposite effects of antibodies against CD11a (LFA-1-alpha-chain) or CD18 (LFA-1- beta-chain). Here, we determined the effects of anti-CD11a and anti- CD18 mAb on proliferation of cloned influenza virus-specific T cells. Anti-CD18 mAb had similar inhibiting effects on the proliferative response of T cell clones induced by immobilized anti-CD3 mAb as it had on the response of peripheral blood T cells. In contrast to its costimulatory effect on resting peripheral blood T cells, anti-CD11a mAb did not increase the proliferation of cloned T cells. Similar differences in effects of anti-CD11a and anti-CD18 mAb were observed when proliferation of the T cell clones was induced by immobilized anti- TCR mAb. When proliferation was induced by influenza virus presented by monocytes as APC, both anti-CD11a and anti-CD18 mAb inhibited T cell proliferation. However, when EBV-transformed B cells were used as APC, neither anti-CD11a nor anti-CD18 mAb inhibited proliferation. These results demonstrate that the effects of antibodies against CD11a (LFA-1- alpha) or CD18 (LFA-1-beta) on T cell proliferation depend on 1) the stage of activation of the T cells, 2) the activation stimulus and its requirement for intercellular adhesion involving LFA-1, and 3) the type of cell used to present Ag.


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
J.-J. Goval, R. Greimers, J. Boniver, and L. de Leval
Germinal Center Dendritic Cells Express More ICAM-1 Than Extrafollicular Dendritic Cells and ICAM-1/LFA-1 Interactions are Involved in the Capacity of Dendritic Cells to Induce PBMCs Proliferation
J. Histochem. Cytochem., January 1, 2006; 54(1): 75 - 84.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Salomon and J. A. Bluestone
Cutting Edge: LFA-1 Interaction with ICAM-1 and ICAM-2 Regulates Th2 Cytokine Production
J. Immunol., November 15, 1998; 161(10): 5138 - 5142.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Champe, B. W. McIntyre, and P. W. Berman
Monoclonal Antibodies That Block the Activity of Leukocyte Function-associated Antigen 1 Recognize Three Discrete Epitopes in the Inserted Domain of CD11a
J. Biol. Chem., January 20, 1995; 270(3): 1388 - 1394.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.