| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 144, Issue 8 3130-3136, Copyright © 1990 by American Association of Immunologists
ARTICLES |
TM Jung and MO Dailey
Department of Pathology, University of Iowa, College of Medicine, Iowa City 52242.
Lymphocyte entry into lymph nodes and Peyer's patches is initiated by the adhesion of the lymphocytes to specialized postcapillary high endothelial venules (HEV). The binding of lymphocytes to lymph node HEV is mediated by the cell surface receptor gp90MEL-14 (gp90). Previous work has shown that gp90 is down-regulated over a period of days after mitogenic or mixed lymphocyte reaction stimulation of T lymphocytes. In our study, it is shown that stimulation of lymphocytes with activators of protein kinase C (PKC), such as PMA or 1-oleoyl 2-acetyl-glycerol, results in the nearly complete loss of surface expression of gp90 within 1 h. Pretreatment of the cells with H-7 or staurosporine, PKC inhibitors, but not HA1004, a general protein kinase inhibitor, prevents the loss of gp90MEL-14. Within 15 min of stimulation of PKC, a novel form of gp90 can be immunoprecipitated from the supernatant of stimulated cells. Upon deglycosylation, this soluble gp90 polypeptide is shown to be 12 kDa smaller than the cell surface protein. Peptide mapping showed identical patterns for surface and soluble receptor, confirming that the soluble Ag is related to the cell membrane protein. Together, these experiments suggest that activation of PKC results in the proteolytic cleavage of gp90MEL-14, resulting in receptor shedding and the inability of the lymphocytes to adhere to HEV endothelium. Furthermore, because supernatant from unstimulated, normal lymphocytes also contains a small amount of the low Mr form of gp90, cell surface proteolysis may be part of the normal turnover of this receptor glycoprotein. These experiments suggest that PKC may play a role in the regulation of lymphocyte traffic to lymphoid tissues.
This article has been cited by other articles:
|
|
 |
|
  V. A. Resto, M. M. Burdick, N. M. Dagia, S. D. McCammon, S. M. Fennewald, and R. Sackstein L-selectin-mediated Lymphocyte-Cancer Cell Interactions under Low Fluid Shear Conditions J. Biol. Chem., June 6, 2008; 283(23): 15816 - 15824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Guezguez, P. Vigneron, N. Lamerant, C. Kieda, T. Jaffredo, and D. Dunon Dual Role of Melanoma Cell Adhesion Molecule (MCAM)/CD146 in Lymphocyte Endothelium Interaction: MCAM/CD146 Promotes Rolling via Microvilli Induction in Lymphocyte and Is an Endothelial Adhesion Receptor J. Immunol., November 15, 2007; 179(10): 6673 - 6685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Reichardt, B. Dornbach, S. Rong, S. Beissert, F. Gueler, K. Loser, and M. Gunzer Naive B cells generate regulatory T cells in the presence of a mature immunologic synapse Blood, September 1, 2007; 110(5): 1519 - 1529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Badr, G. Borhis, D. Treton, C. Moog, O. Garraud, and Y. Richard HIV Type 1 Glycoprotein 120 Inhibits Human B Cell Chemotaxis to CXC Chemokine Ligand (CXCL) 12, CC Chemokine Ligand (CCL)20, and CCL21 J. Immunol., July 1, 2005; 175(1): 302 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Shpacovitch, G. Varga, A. Strey, M. Gunzer, F. Mooren, J. Buddenkotte, N. Vergnolle, C. P. Sommerhoff, S. Grabbe, V. Gerke, et al. Agonists of proteinase-activated receptor-2 modulate human neutrophil cytokine secretion, expression of cell adhesion molecules, and migration within 3-D collagen lattices J. Leukoc. Biol., August 1, 2004; 76(2): 388 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Galkina, K. Tanousis, G. Preece, M. Tolaini, D. Kioussis, O. Florey, D. O. Haskard, T. F. Tedder, and A. Ager L-Selectin Shedding Does Not Regulate Constitutive T Cell Trafficking but Controls the Migration Pathways of Antigen-activated T Lymphocytes J. Exp. Med., November 3, 2003; 198(9): 1323 - 1335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Frenette, N. Chbinou, C. Godbout, D. Marsolais, and P. S. Frenette Macrophages, not neutrophils, infiltrate skeletal muscle in mice deficient in P/E selectins after mechanical reloading Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2003; 285(4): R727 - R732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Walcheck, S. R. Alexander, C. A. St. Hill, and E. Matala ADAM-17-independent shedding of L-selectin J. Leukoc. Biol., September 1, 2003; 74(3): 389 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Fors, K. Goodarzi, and U. H. von Andrian L-Selectin Shedding Is Independent of Its Subsurface Structures and Topographic Distribution J. Immunol., October 1, 2001; 167(7): 3642 - 3651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. L. Wormley Jr., J. Chaiban, and P. L. Fidel Jr. Cell Adhesion Molecule and Lymphocyte Activation Marker Expression during Experimental Vaginal Candidiasis Infect. Immun., August 1, 2001; 69(8): 5072 - 5079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sackstein and C. J. Dimitroff A hematopoietic cell L-selectin ligand that is distinct from PSGL-1 and displays N-glycan-dependent binding activity Blood, October 15, 2000; 96(8): 2765 - 2774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Borland, G. Murphy, and A. Ager Tissue Inhibitor of Metalloproteinases-3 Inhibits Shedding of L-selectin from Leukocytes J. Biol. Chem., January 29, 1999; 274(5): 2810 - 2815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. M. Dong, L. Jackson, and J. W. Murphy Mechanisms for Induction of L-Selectin Loss from T Lymphocytes by a Cryptococcal Polysaccharide, Glucuronoxylomannan Infect. Immun., January 1, 1999; 67(1): 220 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. C. Romzek, E. S. Harris, C. L. Dell, J. Skronek, E. Hasse, P. J. Reynolds, S. W. Hunt III, and Y. Shimizu Use of a beta 1 Integrin-deficient Human T Cell to Identify beta 1 Integrin Cytoplasmic Domain Sequences Critical for Integrin Function Mol. Biol. Cell, October 1, 1998; 9(10): 2715 - 2727. [Abstract] [Full Text]
|
|
|
|
|
|
B. Gu, L.J. Bendall, and J.S. Wiley Adenosine Triphosphate-Induced Shedding of CD23 and L-Selectin (CD62L) From Lymphocytes Is Mediated by the Same Receptor but Different Metalloproteases Blood, August 1, 1998; 92(3): 946 - 951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Mariscalco, M. H. Tcharmtchi, and C. W. Smith P-Selectin Support of Neonatal Neutrophil Adherence Under Flow: Contribution of L-Selectin, LFA-1, and Ligand(s) for P-Selectin Blood, June 15, 1998; 91(12): 4776 - 4785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M G Macey, D A McCarthy, U H Trivedi, G E Venn, D J Chambers, and K A Brown Neutrophil adhesion molecule expression during cardiopulmonary bypass: a comparative study of roller and centrifugal pumps Perfusion, September 1, 1997; 12(5): 293 - 301. [Abstract] [PDF]
|
|
|
|
|
|
B. Haribabu, D. A. Steeber, H. Ali, R. M. Richardson, R. Snyderman, and T. F. Tedder Chemoattractant Receptor-induced Phosphorylation of L-selectin J. Biol. Chem., May 23, 1997; 272(21): 13961 - 13965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Preece, G. Murphy, and A. Ager Metalloproteinase-mediated Regulation of L-selectin Levels on Leucocytes J. Biol. Chem., May 17, 1996; 271(20): 11634 - 11640. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Feehan, K. Darlak, J. Kahn, B. Walcheck, A. F. Spatola, and T. K. Kishimoto Shedding of the Lymphocyte L-Selectin Adhesion Molecule Is Inhibited by a Hydroxamic Acid-based Protease Inhibitor J. Biol. Chem., March 22, 1996; 271(12): 7019 - 7024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Mueller, W. P. Schraw, and A. Richmond Activation of Protein Kinase C Enhances the Phosphorylation of the Type B Interleukin-8 Receptor and Stimulates Its Degradation in Non-hematopoietic Cells J. Biol. Chem., May 5, 1995; 270(18): 10439 - 10448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lasky Selectins: interpreters of cell-specific carbohydrate information during inflammation Science, November 6, 1992; 258(5084): 964 - 969. [Abstract] [PDF]
|
|
|
|
|
|
L.-c. Zhao, M. Shey, M. Farnsworth, and M. O. Dailey Regulation of Membrane Metalloproteolytic Cleavage of L-selectin (CD62L) by the Epidermal Growth Factor Domain J. Biol. Chem., August 10, 2001; 276(33): 30631 - 30640. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
