The Journal of Immunology, Vol 144, Issue 8 2940-2945, Copyright © 1990 by American Association of Immunologists

ARTICLES

Murine B cells expressing Thy-1 after in vivo immunization selectively secrete IgE

CM Snapper, JJ Hooley, S Barbieri and FD Finkelman
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.

IL-4 induces Thy-1 expression and IgE secretion by LPS--and T cell- stimulated murine B cells in vitro. IFN-gamma inhibits both of these IL- 4-mediated effects. IL-4 and IFN-gamma are often exclusively produced by different CD4+ T cell subsets. Injection of mice with a polyclonal goat anti-mouse IgD antibody (GaM delta) stimulates large increases in the serum concentration of IgE through the production of IL-4. Neutralization of endogenous IFN-gamma production in GaM delta-injected mice leads to further increases in serum IgE levels. We show that IL-4 and IFN-gamma, produced after GaM delta injection, respectively, stimulate and inhibit the number of splenic B cells expressing Thy-1 in vivo. Increased numbers of Thy-1-expressing B cells are observed concomitantly with the onset of enhanced IgE secretion. These Thy-1- expressing B cells are highly and selectively enriched for IgE- secreting cells.

This article has been cited by other articles:

				S. M. Kerfoot, M. Szczepanik, J. W. Tung, and P. W. Askenase Identification of Initiator B Cells, a Novel Subset of Activation-Induced Deaminase-Dependent B-1-Like Cells That Mediate Initiation of Contact Sensitivity J. Immunol., August 1, 2008; 181(3): 1717 - 1727. [Abstract] [Full Text] [PDF]