The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rosenkrantz, K.
Right arrow Articles by Flomenberg, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rosenkrantz, K.
Right arrow Articles by Flomenberg, N.

The Journal of Immunology, Vol 144, Issue 5 1721-1728, Copyright © 1990 by American Association of Immunologists

ARTICLES

Both ongoing suppression and clonal elimination contribute to graft- host tolerance after transplantation of HLA mismatched T cell-depleted marrow for severe combined immunodeficiency

K Rosenkrantz, C Keever, K Bhimani, A Horvath, J Brochstein, R O'Reilly, B Dupont and N Flomenberg
Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.

Lymphocytes from children with severe combined immunodeficiency who had been immunologically reconstituted with haploidentical T cell-depleted bone marrow were analyzed with regard to their immunologic recognition of donor, host, or third party alloantigens. When compared with freshly isolated donor lymphocytes, the engrafted donor cells exhibited markedly reduced to absent responses toward host Ag in primary or secondary MLC and cell-mediated lympholysis assays. However, under limiting dilution conditions, cytotoxic responses to host Ag could be demonstrated, indicating that small numbers of host reactive cells were present, although down-regulated at high responder cell doses. These results are consistent with prior observations in limiting dilution cultures that indicate that cells with the potential to lyse autologous target cells exist in the peripheral blood of all normal individuals. The number of host reactive cells present in these patients is significantly less than that present in cells isolated directly from the marrow donors, and is also less than the number of autocytotoxic cells normally seen in peripheral blood. Together, these observations indicate that two mechanisms contribute to donor host tolerance in these patients. The majority of host reactive cells appear to have undergone clonal deletion or inactivation, whereas the small residual host-reactive population appears to be under ongoing immunoregulatory control.


This article has been cited by other articles:


Home page
 NEJMHome page
E. R. Stiehm, R. L. Roberts, J. Hanley-Lopez, M. E. Wakim, M. G. Pallavicini, M. J. Cowan, R. B. Ettenger, and S. A. Feig
Bone Marrow Transplantation in Severe Combined Immunodeficiency from a Sibling Who Had Received a Paternal Bone Marrow Transplant
N. Engl. J. Med., December 12, 1996; 335(24): 1811 - 1814.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.