The Journal of Immunology, Vol 144, Issue 4 1411-1417, Copyright © 1990 by American Association of Immunologists

ARTICLES

Studies on molecular regulation of phagocytosis and activation of the NADPH oxidase in neutrophils. IgG- and C3b-mediated ingestion and associated respiratory burst independent of phospholipid turnover and Ca2+ transients

V Della Bianca, M Grzeskowiak and F Rossi
Instituto di Patologia Generale, Universita di Verona, Italy.

The role of messengers derived from hydrolysis of phosphoinositides and other phospholipids, of the basal level of [Ca2+]i and of the increase in [Ca2+]i in phagocytosis and respiratory burst was investigated, using normal neutrophils and neutrophils Ca2(+)-depleted by pretreatment with Quin2/AM and EGTA. 1) Phagocytosis and respiratory burst in control neutrophils challenged with yeast opsonized with IgG or C3b/bi were associated with a stimulation of the production of inositol phosphates, diacylglycerol, phosphatidic acid, arachidonic acid, and rise in [Ca2+]i. 2) In Ca2(+)-depleted neutrophils (basal [Ca2+]i 10 to 20 nM) the phagocytosis of yeast-IgG was similar to that in control neutrophils, the respiratory burst was slightly depressed (- 30%), while the increase in [Ca2+]i and production of inositol phosphates, diacylglycerol, and phosphatidic and arachidonic acid did not occur. 3) In Ca2(+)-depleted neutrophils the phagocytosis of yeast- C3b/bi was slightly lower than that in control neutrophils, and the respiratory burst, related to the same number of particles ingested, was depressed by about 60%, whereas the increase in [Ca2+]i and production of inositol phosphates, diacylglycerol, phosphatidic acid, and arachidonic acid release did not occur. These findings demonstrate that transmembrane signaling pathways involving the hydrolysis of phosphoinositides by phospholipase C and D and of other phospholipids by phospholipase C and Az, and the rise in [Ca2+]i are not essential processes for triggering the ingestion of yeast particles opsonized with IgG and C3b/bi and the activation of the NADPH oxidase.

This article has been cited by other articles:

				J. Gomez-Cambronero, M. Di Fulvio, and K. Knapek Understanding phospholipase D (PLD) using leukocytes: PLD involvement in cell adhesion and chemotaxis J. Leukoc. Biol., August 1, 2007; 82(2): 272 - 281. [Abstract] [Full Text] [PDF]

				D. Lissandrini, W. Vermi, M. Vezzalini, S. Sozzani, F. Facchetti, G. Bellone, A. Mafficini, F. Gentili, M. G. Ennas, C. Tecchio, et al. Receptor-type protein tyrosine phosphatase gamma (PTP{gamma}), a new identifier for myeloid dendritic cells and specialized macrophages Blood, December 15, 2006; 108(13): 4223 - 4231. [Abstract] [Full Text] [PDF]

				K. L. Cheeseman, T. Ueyama, T. M. Michaud, K. Kashiwagi, D. Wang, L. A. Flax, Y. Shirai, D. J. Loegering, N. Saito, and M. R. Lennartz Targeting of Protein Kinase C-{epsilon} during Fc{gamma} Receptor-dependent Phagocytosis Requires the {epsilon}C1B Domain and Phospholipase C-{gamma}1 Mol. Biol. Cell, February 1, 2006; 17(2): 799 - 813. [Abstract] [Full Text] [PDF]

				P. J. Tacken, K. L. Hartshorn, M. R. White, C. van Kooten, J. G. J. van de Winkel, K. B. M. Reid, and J. J. Batenburg Effective Targeting of Pathogens to Neutrophils via Chimeric Surfactant Protein D/Anti-CD89 Protein J. Immunol., April 15, 2004; 172(8): 4934 - 4940. [Abstract] [Full Text] [PDF]

				E. Garcia-Garcia and C. Rosales Signal transduction during Fc receptor-mediated phagocytosis J. Leukoc. Biol., December 1, 2002; 72(6): 1092 - 1108. [Abstract] [Full Text] [PDF]

				M. Majeed, E. Caveggion, C. A. Lowell, and G. Berton Role of Src kinases and Syk in Fc{gamma} receptor-mediated phagocytosis and phagosome-lysosome fusion J. Leukoc. Biol., November 1, 2001; 70(5): 801 - 811. [Abstract] [Full Text] [PDF]

				R. May and L. Machesky Phagocytosis and the actin cytoskeleton J. Cell Sci., January 3, 2001; 114(6): 1061 - 1077. [Abstract] [PDF]

				J. C. Edberg, C.-T. Lin, D. Lau, J. C. Unkeless, and R. P. Kimberly The Ca[IMAGE] Dependence of Human Fc[IMAGE] Receptor-initiated Phagocytosis J. Biol. Chem., September 22, 1995; 270(38): 22301 - 22307. [Abstract] [Full Text] [PDF]