The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Monostori, E.
Right arrow Articles by Crumpton, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Monostori, E.
Right arrow Articles by Crumpton, M. J.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*L-TYROSINE

The Journal of Immunology, Vol 144, Issue 3 1010-1014, Copyright © 1990 by American Association of Immunologists

ARTICLES

Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor zeta-chains

E Monostori, D Desai, MH Brown, DA Cantrell and MJ Crumpton
Cell Surface Biochemistry, Imperial Cancer Research Fund, London, UK.

The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T11(2) and T11(3), PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 gamma-chain. The combination of T11(2) and T11(3) mAb also induced phosphorylation of the TCR zeta-chain. The phosphorylated zeta- polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T11(2) and T11(3) mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 gamma-chain and the tyrosine phosphorylation of the zeta-chain, respectively.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. I. Rodriguez, N. Girones, and M. Fresno
Cha, a Basic Helix-Loop-Helix Transcription Factor Involved in the Regulation of Upstream Stimulatory Factor Activity
J. Biol. Chem., October 31, 2003; 278(44): 43135 - 43145.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. P. Martelli, J. Boomer, M. Bu, and B. E. Bierer
T Cell Regulation of p62dok (Dok1) Association with Crk-L
J. Biol. Chem., November 30, 2001; 276(49): 45654 - 45661.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Meinl, D. Lengenfelder, N. Blank, R. Pirzer, L. Barata, and C. Hivroz
Differential Requirement of ZAP-70 for CD2-Mediated Activation Pathways of Mature Human T Cells
J. Immunol., October 1, 2000; 165(7): 3578 - 3583.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. M. Carmo, M. A. A. Castro, and F. A. Arosa
CD2 and CD3 Associate Independently with CD5 and Differentially Regulate Signaling Through CD5 in Jurkat T Cells
J. Immunol., October 15, 1999; 163(8): 4238 - 4245.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. le Gouvello, V. Manceau, and A. Sobel
Serine 16 of Stathmin as a Cytosolic Target for Ca2+/Calmodulin-Dependent Kinase II After CD2 Triggering of Human T Lymphocytes
J. Immunol., August 1, 1998; 161(3): 1113 - 1122.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.