The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tomita, Y.
Right arrow Articles by Nomoto, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tomita, Y.
Right arrow Articles by Nomoto, K.

The Journal of Immunology, Vol 144, Issue 2 463-473, Copyright © 1990 by American Association of Immunologists

ARTICLES

Importance of suppressor T cells in cyclophosphamide-induced tolerance to the non-H-2-encoded alloantigens. Is mixed chimerism really required in maintaining a skin allograft tolerance?

Y Tomita, H Mayumi, M Eto and K Nomoto
Department of Immunology, Kyushu University Fukuoka, Japan.

Mechanisms of cyclophosphamide (CP)-induced tolerance were studied. When AKR/J Sea (AKR: H-2k) mice were primed i.v. with 5 x 10(7) spleen cells plus 1 x 10(7) bone marrow cells from [C57BL/6 Slc (B6; H-2b) x C3H/He Slc (C3H; H-2k)]F1 (B6C3F1) mice and treated i.p. with 200 mg/kg CP 2 days later, the survival of C3H skin was moderately prolonged, but the survival of either B6 or B6C3F1 skin was not prolonged. By this treatment, however, mixed chimerism of B6C3F1 cells in the AKR mice was not established. When C3H cells were used as the tolerogen, a minimal degree of mixed chimerism associated with profound tolerance to C3H skin was established. Similar results were observed in various donor- recipient combinations. When C3H skin was grafted in the AKR mice 12 wk after the treatment with C3H cells and CP, or B6C3F1 cells and CP, survival of the grafted C3H skin was prolonged remarkably or moderately, respectively, although mixed chimerism was not detectable at the timing of grafting in either of the groups. In this late stage of tolerance, a strong level of tolerogen-specific suppressor cell activity was observed in those tolerant AKR mice. The suppressor activity was mainly attributable to T cells. These results suggest that the role of Ts cells in order to maintain skin tolerance is important in our CP-induced tolerance system, especially in the late stage of tolerance. Moreover, the generation of the Ts cells does not necessarily require the establishment of a long term mixed chimeric state.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
T. Iwai, Y. Tomita, S. Okano, I. Shimizu, Y. Yasunami, T. Kajiwara, Y. Yoshikai, M. Taniguchi, K. Nomoto, and H. Yasui
Regulatory Roles of NKT Cells in the Induction and Maintenance of Cyclophosphamide-Induced Tolerance
J. Immunol., December 15, 2006; 177(12): 8400 - 8409.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Tomita, M. Yoshikawa, Q.-W. Zhang, I. Shimizu, S. Okano, T. Iwai, H. Yasui, and K. Nomoto
Induction of Permanent Mixed Chimerism and Skin Allograft Tolerance Across Fully MHC-Mismatched Barriers by the Additional Myelosuppressive Treatments in Mice Primed with Allogeneic Spleen Cells Followed by Cyclophosphamide
J. Immunol., July 1, 2000; 165(1): 34 - 41.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Takeuchi, M. Inaba, S. Miyashima, R. Ogawa, and S. Ikehara
A New Strategy for Treatment of Autoimmune Diseases in Chimeric Resistant MRL/lpr Mice
Blood, June 15, 1998; 91(12): 4616 - 4623.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.