The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Portanova, J. P.
Right arrow Articles by Kotzin, B. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Portanova, J. P.
Right arrow Articles by Kotzin, B. L.

The Journal of Immunology, Vol 144, Issue 12 4633-4640, Copyright © 1990 by American Association of Immunologists

ARTICLES

Histone autoantigens in murine lupus. Definition of a major epitope within an accessible region of chromatin

JP Portanova, JC Cheronis, JK Blodgett and BL Kotzin
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.

In SLE and in the (NZB x NZW)F1 murine model of this disease, IgG autoantibodies are frequently produced to DNA and histones. In the present study, we define a linear epitope on histone H2B that is recognized by (NZB x NZW)F1 mice. IgG antibodies from anti-H2B positive (but not anti-H2B negative) mice bound strongly to a peptide containing the first 15 N-terminal amino acids, a region that is exposed in chromatin. Competitive inhibition studies showed that the binding of autoantibodies to H2B in ELISA as well as the binding to soluble H2B was substantially blocked by this peptide. Studies with smaller peptides mapped the epitope to residues 3-12. Individual mice recognized different residues within this region, and a sequence search did not reveal proteins other than H2B that could elicit this spectrum of antibodies. Interestingly, these autoantibody specificities were not a component of those induced in preautoimmune mice by immunization with H2B/RNA complexes or with H2B peptide 1-30 containing the autoantigenic sequence. These findings argue that recognition of a specific N- terminal region of self histone contributes to the anti-H2B autoantibody response in lupus. Autoreactive B cells with specificity for this sequence seem to develop only after the autoimmune process has been initiated.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
P. Decker, A. Le Moal, J.-P. Briand, and S. Muller
Identification of a Minimal T Cell Epitope Recognized by Antinucleosome Th Cells in the C-Terminal Region of Histone H4
J. Immunol., July 15, 2000; 165(2): 654 - 662.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Monestier, P. Decker, J.-P. Briand, J. L. Gabriel, and S. Muller
Molecular and Structural Properties of Three Autoimmune IgG Monoclonal Antibodies to Histone H2B
J. Biol. Chem., April 28, 2000; 275(18): 13558 - 13563.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1990 by The American Association of Immunologists, Inc. All rights reserved.