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The Journal of Immunology, Vol 144, Issue 10 3856-3861, Copyright © 1990 by American Association of Immunologists

ARTICLES

Clonotypes of anti-DNA antibodies expressing specific idiotypes in immune complexes of patients with active lupus nephritis

T Muryoi, T Sasaki, A Hatakeyama, S Shibata, M Suzuki, J Seino and K Yoshinaga
Second Department of Internal Medicine, Tohoku University School of Medicine, Seiryocho, Japan.

IEF, using 6 M urea, provides a unique opportunity to analyze the spectrotypes of antibodies in immune complexes (IC) in vivo. Using this technique, we have analyzed the clonotypes of anti-DNA antibodies expressing specific Id in the circulating IC of patients with active lupus nephritis. Serum anti-ssDNA and anti-dsDNA antibodies showed heterogeneous spectrotypes. The antibodies isolated from circulating IC had a restricted clonotype and a neutral charge and were directed mainly to ssDNA and, to a lesser extent, to dsDNA. These samples failed to form complexes with DNA when they were subjected to absorption to a DNA-coupled Sepharose column. Anti-DNA antibodies expressed specific Id, termed O-81 or NE-1, which were detected only in the IC of patients with active lupus nephritis. Anti-DNA clonotypes, including O-81 and NE- 1 idiotypes, were also found in the eluates of renal glomeruli of lupus patients. These results indicate that subpopulations of anti-DNA antibodies in circulating IC are limited, and may play an important role in the pathogenesis of lupus nephritis.


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Y. Suzuki, T. Funato, Y. Munakata, K. Sato, Y. Hirabayashi, T. Ishii, N. Takasawa, T. Ootaka, T. Saito, and T. Sasaki
Chemically Modified Ribozyme to V Gene Inhibits Anti-DNA Production and the Formation of Immune Deposits Caused by Lupus Lymphocytes
J. Immunol., November 15, 2000; 165(10): 5900 - 5905.
[Abstract] [Full Text] [PDF]


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