The Journal of Immunology, Vol 144, Issue 10 3835-3840, Copyright © 1990 by American Association of Immunologists

ARTICLES

Differential regulation of complement factor H and C3 production in human umbilical vein endothelial cells by IFN-gamma and IL-1

RA Brooimans, AA van der Ark, WA Buurman, LA van Es and MR Daha
Department of Nephrology, University Hospital, Leiden, The Netherlands.

Human umbilical vein endothelial cells (HUVEC) synthesize and secrete C component factors H and C3. Addition of T cell growth factor to HUVEC enhanced factor H production, and caused a profound increase in C3 production. In the present study, investigations were initiated to characterize the effects of purified rIL-1 and rIFN-gamma on the production of factor H and C3 at the protein and mRNA level. IFN-gamma enhanced factor H production in a dose-dependent fashion and a two-fold increase was observed with an optimal dose of 200 U/ml, whereas IFN- gamma had no effect on C3 production. IL-1 inhibited factor H secretion, but the production of C3 was increased 10-fold at an optimal dose of 500 pg/ml of IL-1. Kinetic experiments demonstrated that addition of IL-1 to HUVEC resulted in an induction of C3 production after more than 24 h, whereas IFN-gamma already had a significant effect on factor H production after 8 h of culture. IL-1 in combination with IFN-gamma had a synergistic effect on C3 production. The effects of IL-1 and IFN-gamma on factor H and C3 production by HUVEC could be blocked by using neutralizing amounts of antibodies specific for these cytokines. Northern blot analysis showed that factor H mRNA expression was enhanced in IFN-gamma-treated HUVEC and C3 mRNA was induced in IL-1- treated HUVEC, indicating that the observed increase of factor H and C3 probably is controlled by enhancement of transcription or stability of the transcript.

This article has been cited by other articles:

				R. Purwar, M. Wittmann, J. Zwirner, M. Oppermann, M. Kracht, O. Dittrich-Breiholz, R. Gutzmer, and T. Werfel Induction of C3 and CCL2 by C3a in Keratinocytes: A Novel Autocrine Amplification Loop of Inflammatory Skin Reactions J. Immunol., October 1, 2006; 177(7): 4444 - 4450. [Abstract] [Full Text] [PDF]

				A. Verschoor, M. A. Brockman, D. M. Knipe, and M. C. Carroll Cutting Edge: Myeloid Complement C3 Enhances the Humoral Response To Peripheral Viral Infection J. Immunol., September 1, 2001; 167(5): 2446 - 2451. [Abstract] [Full Text] [PDF]

				D. B. Cines, E. S. Pollak, C. A. Buck, J. Loscalzo, G. A. Zimmerman, R. P. McEver, J. S. Pober, T. M. Wick, B. A. Konkle, B. S. Schwartz, et al. Endothelial Cells in Physiology and in the Pathophysiology of Vascular Disorders Blood, May 15, 1998; 91(10): 3527 - 3561. [Full Text] [PDF]

				M. B. Fischer, M. Ma, N. C. Hsu, and M. C. Carroll Local Synthesis of C3 Within the Splenic Lymphoid Compartment Can Reconstitute the Impaired Immune Response in C3-Deficient Mice J. Immunol., March 15, 1998; 160(6): 2619 - 2625. [Abstract] [Full Text] [PDF]