The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lehn, M.
Right arrow Articles by Remold, H. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lehn, M.
Right arrow Articles by Remold, H. G.

The Journal of Immunology, Vol 143, Issue 9 3020-3024, Copyright © 1989 by American Association of Immunologists

ARTICLES

IL-4 inhibits H2O2 production and antileishmanial capacity of human cultured monocytes mediated by IFN-gamma

M Lehn, WY Weiser, S Engelhorn, S Gillis and HG Remold
Department of Rheumatology and Immunology, Brigham and Women's Hospital, Boston, MA 02115.

The effect of IL-4 on the IFN-gamma-induced state of activation of cultured human monocytes was investigated with regard to their ability to produce hydrogen peroxide and their antileishmanial capacity towards the intracellular parasite Leishmania donovani. IL-4 was found to inhibit the IFN-gamma-dependent hydrogen peroxide production of monocytes. Treatment of monocytes with IFN-gamma (200 to 600 U/ml) for 48 h increased the hydrogen peroxide production fourfold above background. Coincubation of the monocytes with IL-4 (1 to 1000 U/ml) and IFN-gamma (200 to 600 U/ml) inhibited this increase by 50 to 100%. IL-4 alone did not modulate the hydrogen peroxide production of monocytes. Pretreatment of monocytes with IL-4 for 20 min to 3 h was already effective in preventing the IFN-gamma response. Addition of IL- 4 not later than 6 h after the start of incubation with IFN-gamma was necessary for an optimal inhibitory effect. IL-4 also inhibited the IFN- gamma-induced antileishmanial capacity of monocytes: IFN-gamma (1000 U/ml) induced a 54 +/- 10% reduction in the number of parasites. Monocytes treated with combinations of IL-4 (100 to 1000 U/ml) and IFN- gamma (1000 U/ml) were unable to reduce the parasite numbers. IL-4 alone did not alter the uptake of Leishmania donovani nor induce antileishmanial activity. These results demonstrate that IL-4 disables human cultured monocytes to respond to IFN-gamma activation.


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
K. Schroder, P. J. Hertzog, T. Ravasi, and D. A. Hume
Interferon-{gamma}: an overview of signals, mechanisms and functions
J. Leukoc. Biol., February 1, 2004; 75(2): 163 - 189.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. R. Houpt, D. J. Glembocki, T. G. Obrig, C. A. Moskaluk, L. A. Lockhart, R. L. Wright, R. M. Seaner, T. R. Keepers, T. D. Wilkins, and W. A. Petri Jr.
The Mouse Model of Amebic Colitis Reveals Mouse Strain Susceptibility to Infection and Exacerbation of Disease by CD4+ T Cells
J. Immunol., October 15, 2002; 169(8): 4496 - 4503.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Pilette, Y. Ouadrhiri, J. Van Snick, J.-C. Renauld, P. Staquet, J.-P. Vaerman, and Y. Sibille
IL-9 Inhibits Oxidative Burst and TNF-{alpha} Release in Lipopolysaccharide-Stimulated Human Monocytes Through TGF-{beta}
J. Immunol., April 15, 2002; 168(8): 4103 - 4111.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. Musso, S. Deaglio, L. Franco, L. Calosso, R. Badolato, G. Garbarino, U. Dianzani, and F. Malavasi
CD38 expression and functional activities are up-regulated by IFN-{gamma} on human monocytes and monocytic cell lines
J. Leukoc. Biol., April 1, 2001; 69(4): 605 - 612.
[Abstract] [Full Text]

Home page
 ThoraxHome page
C. N Serhan and C. B Clish
Aspirin triggered lipid mediators: novel inhibitors of leucocyte trafficking
Thorax, October 1, 2000; 55(90002): 10S - 12.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. A. Munger, A. Montero, M. Fukunaga, S. Uda, T. Yura, E. Imai, Y. Kaneda, J. M. Valdivielso, and K. F. Badr
Transfection of rat kidney with human 15-lipoxygenase suppresses inflammation and preserves function in experimental glomerulonephritis
PNAS, November 9, 1999; 96(23): 13375 - 13380.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Goenka, J. Youn, L. M. Dzurek, U. Schindler, L.-y. Yu-Lee, and M. Boothby
Paired Stat6 C-Terminal Transcription Activation Domains Required Both for Inhibition of an IFN-Responsive Promoter and Trans-Activation
J. Immunol., November 1, 1999; 163(9): 4663 - 4672.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. Hachicha, M. Pouliot, N. A. Petasis, and C. N. Serhan
Lipoxin (LX)A4 and Aspirin-triggered 15-epi-LXA4 Inhibit Tumor Necrosis Factor 1alpha -initiated Neutrophil Responses and Trafficking: Regulators of a Cytokine-Chemokine Axis
J. Exp. Med., June 21, 1999; 189(12): 1923 - 1930.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. A. Power, G. Wei, and P. A. Bretscher
Mycobacterial Dose Defines the Th1/Th2 Nature of the Immune Response Independently of Whether Immunization Is Administered by the Intravenous, Subcutaneous, or Intradermal Route
Infect. Immun., December 1, 1998; 66(12): 5743 - 5750.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. L. Mbow, J. A. Bleyenberg, L. R. Hall, and R. G. Titus
Phlebotomus papatasi Sand Fly Salivary Gland Lysate Down-Regulates a Th1, but Up-Regulates a Th2, Response in Mice Infected with Leishmania major
J. Immunol., November 15, 1998; 161(10): 5571 - 5577.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Nasarre, J. L. Krahenbuhl, and T. R. Klei
Down Regulation of Macrophage Activation in Brugia pahangi-Infected Jirds (Meriones unguiculatus)
Infect. Immun., March 1, 1998; 66(3): 1063 - 1069.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. K. DeKrey, H. C. Lima, and R. G. Titus
Analysis of the Immune Responses of Mice to Infection with Leishmania braziliensis
Infect. Immun., February 1, 1998; 66(2): 827 - 829.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Niiro, T. Otsuka, K. Izuhara, K. Yamaoka, K. Ohshima, T. Tanabe, S. Hara, Y. Nemoto, Y. Tanaka, H. Nakashima, et al.
Regulation by Interleukin-10 and Interleukin-4 of Cyclooxygenase-2 Expression in Human Neutrophils
Blood, March 1, 1997; 89(5): 1621 - 1628.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S.-i. Hashimoto, M. Yamada, K. Motoyoshi, and K. S. Akagawa
Enhancement of Macrophage Colony-Stimulating Factor-Induced Growth and Differentiation of Human Monocytes by Interleukin-10
Blood, January 1, 1997; 89(1): 315 - 321.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Sozzani, C. Cambon, N. Vita, M.-Hélèn. Séguélas, D. Caput, P. Ferrara, and B. Pipy
Interleukin-13 Inhibits Protein Kinase C-triggered Respiratory Burst in Human Monocytes
J. Biol. Chem., March 10, 1995; 270(10): 5084 - 5088.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. Reiner, Z. Wang, F Hatam, P Scott, and R. Locksley
TH1 and TH2 cell antigen receptors in experimental leishmaniasis
Science, March 5, 1993; 259(5100): 1457 - 1460.
[Abstract] [PDF]

Home page
 ScienceHome page
P Salgame, J. Abrams, C Clayberger, H Goldstein, J Convit, R. Modlin, and B. Bloom
Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones
Science, October 11, 1991; 254(5029): 279 - 282.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.