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The Journal of Immunology, Vol 143, Issue 9 2955-2960, Copyright © 1989 by American Association of Immunologists

ARTICLES

The bacterial outer membrane protein that reacts with anti-HLA-B27 antibodies is the OmpA protein

JJ Zhang, M Hamachi, T Hamachi, YP Zhao and DT Yu
Department of Medicine, University of California, Los Angeles 90024- 167022.

A bacterial outer membrane protein of 35-kDa Mr has been reported to react with several anti-HLA-B27 mAb. Here, we demonstrated that this protein showed the heat-modifiability of the OmpA protein during SDS- PAGE. Further, the protein was not detected in mutants of Escherichia coli in which the expression of the OmpA protein has been suppressed. The protein would be reexpressed when one of the mutants was transformed with an expression vector carrying the OmpA gene. Finally, the identity of the reactive protein to OmpA protein was verified by homology in amino acid sequences. An NH2-terminal fragment of this protein was generated by tryptic digestion. Inasmuch as this was unreactive with the anti-HLA-B27 antibody, we concluded that the carboxyl-terminus contributed directly or indirectly to the reactive domain.




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