The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Uchiyama, T.
Right arrow Articles by Yamaura, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uchiyama, T.
Right arrow Articles by Yamaura, N.

The Journal of Immunology, Vol 143, Issue 10 3175-3182, Copyright © 1989 by American Association of Immunologists

ARTICLES

Activation of murine T cells by toxic shock syndrome toxin-1. The toxin- binding structures expressed on murine accessory cells are MHC class II molecules

T Uchiyama, T Tadakuma, K Imanishi, M Araake, S Saito, XJ Yan, H Fujikawa, H Igarashi and N Yamaura
Department of Microbiology, Tokyo Women's Medical College, Japan.

Toxic shock syndrome toxin-1 (TSST-1)-binding structures present on murine lymphoid tissues were investigated by using 125I-TSST-1. T- depleted C57BL/6 spleen cells incubated with TSST-1 for 3 h at 0 degree C were mitogenic to splenic T cells, indicating that the former cells bind and present TSST-1 to T cells. TSST-1-binding activity was observed in C57BL/6 splenic B cells and L cells transfected with I-Ab genes, but not in splenic T cells and control L cells. Scatchard plot analysis showed that these B cells and transfectants bound TSST-1 with similar binding affinity. SDS-PAGE analysis showed that lysates of C57BL/6 spleen cells and the I-Ab-positive transfectants contain a single band which bound TSST-1 and comigrated with I-Ab heterodimers. TSST-1-binding activity observed clearly in C57BL/6. BALB/c, and C3H/HeN spleen cells and L cells transfected with I-Ab or I-Ak genes was not reduced by paraformaldehyde fixation. Binding of 125I-TSST-1 to the three spleen cells was markedly reduced by anti-I-A antibodies, but not by anti-I-E antibodies. C57BL/6, C3H/HeN, and (C3H/HeN x C57BL/6) F1 T cells were activated by TSST-1 to proliferate and produce IL-2 in the presence of FT6.2 cells, LT1-30-3 cells and either of them, respectively, but not in the presence of control L cells. These results indicate that I-A molecules function as the structures via that accessory cells directly bind TSST-1 on the cell surface and present a triggering signal of TSST-1 to T cells.


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
D.-L. Hu, K. Omoe, F. Inoue, T. Kasai, M. Yasujima, K. Shinagawa, and A. Nakane
Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates
J. Med. Microbiol., September 1, 2008; 57(9): 1106 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
P. Sauer, J. Sila, T. Stosova, R. Vecerova, P. Hejnar, I. Vagnerova, M. Kolar, V. Raclavsky, J. Petrzelova, Y. Loveckova, et al.
Prevalence of genes encoding extracellular virulence factors among meticillin-resistant Staphylococcus aureus isolates from the University Hospital, Olomouc, Czech Republic
J. Med. Microbiol., April 1, 2008; 57(4): 403 - 410.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
F. Layer, B. Ghebremedhin, W. Konig, and B. Konig
Heterogeneity of Methicillin-Susceptible Staphylococcus aureus Strains at a German University Hospital Implicates the Circulating-Strain Pool as a Potential Source of Emerging Methicillin-Resistant S. aureus Clones.
J. Clin. Microbiol., June 1, 2006; 44(6): 2179 - 2185.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Wu, J. R. Rodgers, X.-Y. D. Perrard, J. L. Perrard, J. E. Prince, Y. Abe, B. K. Davis, G. Dietsch, C. W. Smith, and C. M. Ballantyne
Deficiency of CD11b or CD11d Results in Reduced Staphylococcal Enterotoxin-Induced T Cell Response and T Cell Phenotypic Changes
J. Immunol., July 1, 2004; 173(1): 297 - 306.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
K. Kikuchi, N. Takahashi, C. Piao, K. Totsuka, H. Nishida, and T. Uchiyama
Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus Strains Causing Neonatal Toxic Shock Syndrome-Like Exanthematous Disease in Neonatal and Perinatal Wards
J. Clin. Microbiol., July 1, 2003; 41(7): 3001 - 3006.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Chen, M. Koyanagi, K. Fukada, K.'i. Imanishi, J. Yagi, H. Kato, T. Miyoshi-Akiyama, R. Zhang, K. Miwa, and T. Uchiyama
Continuous Exposure of Mice to Superantigenic Toxins Induces a High-Level Protracted Expansion and an Immunological Memory in the Toxin-Reactive CD4+ T Cells
J. Immunol., April 15, 2002; 168(8): 3817 - 3824.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Niedergang, A.ès Hémar, C. R. A. Hewitt, M. J. Owen, A. Dautry-Varsat, and A.és Alcover
The Staphylococcus aureus Enterotoxin B Superantigen Induces Specific T Cell Receptor Down-regulation by Increasing Its Internalization
J. Biol. Chem., May 26, 1995; 270(21): 12839 - 12845.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
P Marrack and J Kappler
The staphylococcal enterotoxins and their relatives
Science, May 11, 1990; 248(4956): 705 - 711.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.