The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reed, S. L.
Right arrow Articles by Gigli, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reed, S. L.
Right arrow Articles by Gigli, I.

The Journal of Immunology, Vol 143, Issue 1 189-195, Copyright © 1989 by American Association of Immunologists

ARTICLES

Cleavage of C3 by a neutral cysteine proteinase of Entamoeba histolytica

SL Reed, WE Keene, JH McKerrow and I Gigli
Department of Medicine, UCSD Medical Center, San Diego, CA 92103.

The major secreted proteinase of Entamoeba histolytica, a 56-kDa neutral cysteine proteinase, activates C by cleaving C3. The action of the proteinase is similar to C-derived C3 convertases because it produces a single cleavage of the alpha-chain in a dose- and time- dependent manner and cleaves C3 between residues 78 and 79, only one amino acid residue distal to the natural site acted on by the C3 convertases. C3a generation was detected by RIA. The 105-kDa fragment produced by the cleavage of the alpha-chain was structurally and functionally equivalent to the alpha'-chain of C3b, as demonstrated by susceptibility to the action of factors I and H and participation in the activation of the alternative pathway of C. Activation of C by the 56-kDa neutral cysteine proteinase may play a role in the early inflammatory response in amebic lesions and thus contribute to the pathogenesis of invasive amebiasis.


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
S. G. Melendez-Lopez, S. Herdman, K. Hirata, M.-H. Choi, Y. Choe, C. Craik, C. R. Caffrey, E. Hansell, B. Chavez-Munguia, Y. T. Chen, et al.
Use of Recombinant Entamoeba histolytica Cysteine Proteinase 1 To Identify a Potent Inhibitor of Amebic Invasion in a Human Colonic Model
Eukaryot. Cell, July 1, 2007; 6(7): 1130 - 1136.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. L. Stanley Jr. and S. L. Reed
Microbes and Microbial Toxins: Paradigms for Microbial-Mucosal Interactions: VI. Entamoeba histolytica: parasite-host interactions
Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1049 - G1054.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
X. Que and S. L. Reed
Cysteine Proteinases and the Pathogenesis of Amebiasis
Clin. Microbiol. Rev., April 1, 2000; 13(2): 196 - 206.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
M. Espinosa-Cantellano and A. Martinez-Palomo
Pathogenesis of Intestinal Amebiasis: From Molecules to Disease
Clin. Microbiol. Rev., April 1, 2000; 13(2): 318 - 331.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
A Jansson, F Gillin, U Kagardt, and P Hagblom
Coding of hemolysins within the ribosomal RNA repeat on a plasmid in Entamoeba histolytica
Science, March 11, 1994; 263(5152): 1440 - 1443.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.