The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tanaka, T.
Right arrow Articles by Hashimoto, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanaka, T.
Right arrow Articles by Hashimoto, Y.

The Journal of Immunology, Vol 142, Issue 8 2791-2795, Copyright © 1989 by American Association of Immunologists

ARTICLES

Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody

T Tanaka, T Masuko, H Yagita, T Tamura and Y Hashimoto
Department of Hygienic Chemistry, Tohoku University, Sendai, Japan.

A mouse mAb, which recognized a rat T cell surface Ag responsible for the T cell activation, was produced by a regular hybridoma method using F344 rat T cells stimulated with PMA and a calcium ionophore, as the Ag. The mAb termed 1F4 (kappa-IgM) was reactive with rat T cells but not with B cells and immunohistochemically it stained rat thymus tissues strongly at medulla and weakly rat cortex. Addition of 1F4 mAb to a culture of T cells resulted in the proliferation of T cells by a help of PMA or a solid support. 1F4 mAb also caused the modulation of the corresponding Ag but not other T cell markers such as CD5, CD2, and OX-52-defined Ag. The 1F4 mAb immunoprecipitated a cell surface component having an apparent m.w. of 25,000 from rat T cells which could be associated with a disulfide-linked heterodimer (m.w. 92,000) consists of subunits having m.w. of about 52,000 and 43,000. These results strongly suggest that the 1F4 mAb recognizes a rat T cell Ag homologous to the human and mouse CD3.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Pelegri, J. Vilaplana, C. Castellote, M. Rabanal, A. Franch, and M. Castell
Circadian rhythms in surface molecules of rat blood lymphocytes
Am J Physiol Cell Physiol, January 1, 2003; 284(1): C67 - C76.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Iizuka, T. Tanaka, M. Suematsu, S. Miura, T. Watanabe, R. Koike, Y. Ishimura, H. Ishii, N. Miyasaka, and M. Miyasaka
Stage-Specific Expression of Mucosal Addressin Cell Adhesion Molecule-1 During Embryogenesis in Rats
J. Immunol., March 1, 2000; 164(5): 2463 - 2471.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. F. Stachlewitz, X. Li, S. Smith, H. Bunzendahl, L. M. Graves, and R. G. Thurman
Glycine Inhibits Growth of T Lymphocytes by an IL-2-Independent Mechanism
J. Immunol., January 1, 2000; 164(1): 176 - 182.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Chiba, Y. Yanagawa, Y. Masubuchi, H. Kataoka, T. Kawaguchi, M. Ohtsuki, and Y. Hoshino
FTY720, a Novel Immunosuppressant, Induces Sequestration of Circulating Mature Lymphocytes by Acceleration of Lymphocyte Homing in Rats. I. FTY720 Selectively Decreases the Number of Circulating Mature Lymphocytes by Acceleration of Lymphocyte Homing
J. Immunol., May 15, 1998; 160(10): 5037 - 5044.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Z Prakapas, M Denoyelle, C Dargemont, F. Kroese, J. Thiery, and M. Deugnier
Enrichment and characterization of thymus-repopulating cells in stroma-dependent cultures of rat bone marrow
J. Cell Sci., January 4, 1993; 104(4): 1039 - 1048.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.