The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwarz, R. E.
Right arrow Articles by Hiserodt, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwarz, R. E.
Right arrow Articles by Hiserodt, J. C.

The Journal of Immunology, Vol 141, Issue 10 3318-3323, Copyright © 1988 by American Association of Immunologists

ARTICLES

The expression and functional involvement of laminin-like molecules in non-MHC restricted cytotoxicity by human Leu-19+/CD3- natural killer lymphocytes

RE Schwarz and JC Hiserodt
Pittsburgh Cancer Institute, PA 15213.

We have recently reported a simple technique for the purification of large granular lymphocyte/NK cells with broad anti-tumor cytotoxicity (lymphokine-activated killer (LAK) activity) by the induction of their adherence to plastic surfaces by recombinant IL-2. Using this technique with nylon wool passed human peripheral blood or splenic lymphocytes we generated populations of adherent LAK (A-LAK) cells which were 80 to 95% large granular lymphocyte and up to 97% Leu-19+ cells. Using two- color fluorescence and cell sorting we now demonstrate that these same cells express a high density of surface structures which are immunochemically cross-reactive with affinity purified anti-laminin antibody. Laminin-like structures were expressed on 80 to 90% of Leu- 19+/CD3- LAK cells but were not expressed on resting CD3+/CD4+/Leu19- T cells or CD3+/CD8+/Leu19- T cells. Laminin-like molecules were also expressed on resting Leu19+/CD3- large granular lymphocyte/NK cells (purified to more than 98% by cell sorting) and increased in intensity as these cells developed LAK activity in response to rIL-2. The functional involvement of laminin-like molecules in NK and LAK cytotoxicity was demonstrated by the ability of affinity purified anti- laminin F(ab')2 antibody to inhibit cytolytic activity in a dose- dependent manner (50% inhibition with 10 to 20 micrograms F(ab')2). Analysis of the mechanism of inhibition using single cell cytotoxicity assays indicated that the laminin-like structure was involved in activation of cytotoxicity and not in primary target cell adhesion. We believe that this structure may function as a novel recognition/activation structure used by cells mediating non-MHC restricted cytotoxicity.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
T Geberhiwot, D Assefa, J Kortesmaa, S Ingerpuu, C Pedraza, Z Wondimu, J Charo, R Kiessling, I Virtanen, K Tryggvason, et al.
Laminin-8 (alpha4beta1gamma1) is synthesized by lymphoid cells, promotes lymphocyte migration and costimulates T cell proliferation
J. Cell Sci., January 1, 2001; 114(2): 423 - 433.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1988 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1988 by The American Association of Immunologists, Inc. All rights reserved.