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The Journal of Immunology, Vol 138, Issue 3 732-738, Copyright © 1987 by American Association of Immunologists

ARTICLES

T cell hybridomas reactive with the acetylcholine receptor and its subunits

JA Tami, OE Urso and KA Krolick

A panel of thirty cloned rat-mouse T cell hybridomas was prepared by fusion of acetylcholine receptor (AChR)-reactive rat T cells with the mouse thymoma BW5147. The T cell hybrids were demonstrated to be AChR reactive by their ability to secrete IL 2 in response to either AChR itself or by purified AChR subunits (alpha,beta,gamma, or delta). Various patterns of AChR subunit reactivity were observed, suggesting a predominant recognition of the alpha subunit, and also a considerable cross-reactivity from one subunit to another.




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