The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, Y. X.
Right arrow Articles by Caldwell, H. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, Y. X.
Right arrow Articles by Caldwell, H. D.

The Journal of Immunology, Vol 138, Issue 2 575-581, Copyright © 1987 by American Association of Immunologists

ARTICLES

Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis

YX Zhang, S Stewart, T Joseph, HR Taylor and HD Caldwell

A panel of monoclonal antibodies (MAb) was generated against Chlamydia trachomatis serovar B, an etiologic agent of blinding trachoma. The specificities of MAb were determined by dot blot assay by using viable elementary bodies of 13 C. trachomatis serovars and two C. psittaci strains. The dot blot assay was used to identify those antigens that were unique and immunoaccessible on the chlamydial surface. MAb were identified that recognized bi-specific (serovars B and Ba) or subspecies-specific (various B complex serovars) surface-exposed antigenic determinants that were either resistant or sensitive to heat denaturation (56 degrees C, 30 min). All of the MAb recognized the major outer membrane protein as determined by either immunoblotting or radioimmunoprecipitation. MAb specific for immunoaccessible major outer membrane protein epitopes protected mice from toxic death after i.v. injection of B serovar elementary bodies and neutralized the infectivity of the organism for monkey eyes. In contrast, MAb reactive against non-immunoaccessible subspecies- or species-specific major outer membrane protein epitopes or against an immunoaccessible genus- specific epitope located on chlamydial lipopolysaccharide did not protect mice from toxic death or neutralize infectivity of the parasite for monkey eyes. These data suggest that those major outer membrane protein antigenic determinants that are serovar or serogroup specific and are accessible to antibody on the chlamydial cell surface may be useful as a recombinant subunit vaccine for trachoma.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
D. D. Crane, J. H. Carlson, E. R. Fischer, P. Bavoil, R.-c. Hsia, C. Tan, C.-c. Kuo, and H. D. Caldwell
From the Cover: Chlamydia trachomatis polymorphic membrane protein D is a species-common pan-neutralizing antigen
PNAS, February 7, 2006; 103(6): 1894 - 1899.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. G. Morrison and R. P. Morrison
A Predominant Role for Antibody in Acquired Immunity to Chlamydial Genital Tract Reinfection
J. Immunol., December 1, 2005; 175(11): 7536 - 7542.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. G. Morrison and R. P. Morrison
The Protective Effect of Antibody in Immunity to Murine Chlamydial Genital Tract Reinfection Is Independent of Immunoglobulin A
Infect. Immun., September 1, 2005; 73(9): 6183 - 6186.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Sharma, A. M. Bosnic, J. M. Piper, and G. Zhong
Human Antibody Responses to a Chlamydia-Secreted Protease Factor
Infect. Immun., December 1, 2004; 72(12): 7164 - 7171.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. N. Steele, Z. R. Balsara, and M. N. Starnbach
Hematopoietic Cells Are Required to Initiate a Chlamydia trachomatis-Specific CD8+ T Cell Response
J. Immunol., November 15, 2004; 173(10): 6327 - 6337.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
A. W. Solomon, R. W. Peeling, A. Foster, and D. C. W. Mabey
Diagnosis and Assessment of Trachoma
Clin. Microbiol. Rev., October 1, 2004; 17(4): 982 - 1011.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
D. MABEY and A. W. SOLOMON
APPLICATION OF MOLECULAR TOOLS IN THE CONTROL OF BLINDING TRACHOMA
Am J Trop Med Hyg, November 1, 2003; 69(2007): 11 - 17.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
W. J. Brown, Y. A. W. Skeiky, P. Probst, and D. D. Rockey
Chlamydial Antigens Colocalize within IncA-Laden Fibers Extending from the Inclusion Membrane into the Host Cytosol
Infect. Immun., October 1, 2002; 70(10): 5860 - 5864.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. P. Morrison and H. D. Caldwell
Immunity to Murine Chlamydial Genital Infection
Infect. Immun., June 1, 2002; 70(6): 2741 - 2751.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
D. E. Kawa and R. S. Stephens
Antigenic Topology of Chlamydial PorB Protein and Identification of Targets for Immune Neutralization of Infectivity
J. Immunol., May 15, 2002; 168(10): 5184 - 5191.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Shaw, V. Grund, L. Durling, D. Crane, and H. D. Caldwell
Dendritic Cells Pulsed with a Recombinant Chlamydial Major Outer Membrane Protein Antigen Elicit a CD4+ Type 2 Rather than Type 1 Immune Response That Is Not Protective
Infect. Immun., March 1, 2002; 70(3): 1097 - 1105.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
E. A. Lindquist, J. D. Marks, B. J. Kleba, and R. S. Stephens
Phage-display antibody detection of Chlamydia trachomatis-associated antigens
Microbiology, February 1, 2002; 148(2): 443 - 451.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
U. Dreses-Werringloer, I. Padubrin, H. Zeidler, and L. Kohler
Effects of Azithromycin and Rifampin on Chlamydia trachomatis Infection In Vitro
Antimicrob. Agents Chemother., November 1, 2001; 45(11): 3001 - 3008.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
D. R. Stothard
Use of a Reverse Dot Blot Procedure To Identify the Presence of Multiple Serovars in Chlamydia trachomatis Urogenital Infection
J. Clin. Microbiol., July 1, 2001; 39(7): 2655 - 2659.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Wolf, E. Fischer, D. Mead, G. Zhong, R. Peeling, B. Whitmire, and H. D. Caldwell
Chlamydia pneumoniae Major Outer Membrane Protein Is a Surface-Exposed Antigen That Elicits Antibodies Primarily Directed against Conformation-Dependent Determinants
Infect. Immun., May 1, 2001; 69(5): 3082 - 3091.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. G. Morrison and R. P. Morrison
Resolution of Secondary Chlamydia trachomatis Genital Tract Infection in Immune Mice with Depletion of Both CD4+ and CD8+ T cells
Infect. Immun., April 1, 2001; 69(4): 2643 - 2649.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. S. Hughes, K. M. Shaw, and R. H. Ashley
Mutagenesis and Functional Reconstitution of Chlamydial Major Outer Membrane Proteins: VS4 Domains Are Not Required for Pore Formation but Modify Channel Function
Infect. Immun., March 1, 2001; 69(3): 1671 - 1678.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. Vretou, E. Psarrou, M. Kaisar, I. Vlisidou, V. Salti-Montesanto, and D. Longbottom
Identification of Protective Epitopes by Sequencing of the Major Outer Membrane Protein Gene of a Variant Strain of Chlamydia psittaci Serotype 1 (Chlamydophila abortus)
Infect. Immun., January 1, 2001; 69(1): 607 - 612.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-K. Kim, L. Devine, M. Angevine, R. DeMars, and P. B. Kavathas
Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers
J. Immunol., December 15, 2000; 165(12): 7285 - 7292.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
U. Dreses-Werringloer, I. Padubrin, B. Jürgens-Saathoff, A. P. Hudson, H. Zeidler, and L. Köhler
Persistence of Chlamydia trachomatis Is Induced by Ciprofloxacin and Ofloxacin In Vitro
Antimicrob. Agents Chemother., December 1, 2000; 44(12): 3288 - 3297.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
S. G. Morrison, H. Su, H. D. Caldwell, and R. P. Morrison
Immunity to Murine Chlamydia trachomatis Genital Tract Reinfection Involves B Cells and CD4+ T Cells but Not CD8+ T Cells
Infect. Immun., December 1, 2000; 68(12): 6979 - 6987.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-K. Kim, M. Angevine, K. Demick, L. Ortiz, R. Rudersdorf, D. Watkins, and R. DeMars
Induction of HLA Class I-Restricted CD8+ CTLs Specific for the Major Outer Membrane Protein of Chlamydia trachomatis in Human Genital Tract Infections
J. Immunol., June 1, 1999; 162(11): 6855 - 6866.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. D. Kane, R. M. Vena, S. P. Ouellette, and G. I. Byrne
Intracellular Tryptophan Pool Sizes May Account for Differences in Gamma Interferon-Mediated Inhibition and Persistence of Chlamydial Growth in Polarized and Nonpolarized Cells
Infect. Immun., April 1, 1999; 67(4): 1666 - 1671.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
D. R. Stothard, B. Van Der Pol, N. J. Smith, and R. B. Jones
Effect of Serial Passage in Tissue Culture on Sequence of omp1 from Chlamydia trachomatis Clinical Isolates
J. Clin. Microbiol., December 1, 1998; 36(12): 3686 - 3688.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
D. Vanrompay, E. Cox, J. Mast, B. Goddeeris, and G. Volckaert
High-Level Expression of Chlamydia psittaci Major Outer Membrane Protein in COS Cells and in Skeletal Muscles of Turkeys
Infect. Immun., November 1, 1998; 66(11): 5494 - 5500.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. J. Stagg, M. Tuffrey, C. Woods, E. Wunderink, and S. C. Knight
Protection against Ascending Infection of the Genital Tract by Chlamydia trachomatis Is Associated with Recruitment of Major Histocompatibility Complex Class II Antigen-Presenting Cells into Uterine Tissue
Infect. Immun., August 1, 1998; 66(8): 3535 - 3544.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. R. Stothard, G. Boguslawski, and R. B. Jones
Phylogenetic Analysis of the Chlamydia trachomatis Major Outer Membrane Protein and Examination of Potential Pathogenic Determinants
Infect. Immun., August 1, 1998; 66(8): 3618 - 3625.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Yang and R. C. Brunham
Gene Knockout B Cell-Deficient Mice Demonstrate That B Cells Play an Important Role in the Initiation of T Cell Responses to Chlamydia trachomatis (Mouse Pneumonitis) Lung Infection
J. Immunol., August 1, 1998; 161(3): 1439 - 1446.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
H. Brade, W. Brabetz, L. Brade, O. Hoist, S. Lobau, M. Lucakova, U. Mamat, A. Rozalski, K. Zych, and P. Kosma
Review: Chlamydial lipopolysaccharide
Innate Immunity, February 1, 1997; 4(1): 67 - 84.
[PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1987 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1987 by The American Association of Immunologists, Inc. All rights reserved.