| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 138, Issue 2 566-569, Copyright © 1987 by American Association of Immunologists
ARTICLES |
H Takayama, G Trenn, W Humphrey Jr, JA Bluestone, PA Henkart and MV Sitkovsky
Exocytosis of cytolysin-containing granules from cytotoxic T lymphocytes (CTL) was studied with the use of granule enzyme (BLT esterase) as a convenient biochemical marker. Using cloned CTL, we demonstrate here that BLT esterase secretion into the supernatant is specifically triggered by antigen-bearing target cells and that this secretion is inhibited by soluble monoclonal antibodies against the antigen-specific T cell receptor (TcR). Immobilized anti-receptor antibodies induced efficient enzyme secretion in the absence of target cells, thus implying a direct involvement of TcR complex in triggering exocytosis of granules. These results support the role of the granule exocytosis in CTL functions and provide a quantitative and direct assay of a rapid CTL functional response to antigenic stimulation.
This article has been cited by other articles:
|
|
 |
|
  R Goldbach-Mansky, S Suson, R Wesley, C E Hack, H S El-Gabalawy, and P P Tak Raised granzyme B levels are associated with erosions in patients with early rheumatoid factor positive rheumatoid arthritis Ann Rheum Dis, May 1, 2005; 64(5): 715 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P.-L. Lee, E. Mansfield, S.-C. Hsieh, T. Hernandez-Boussard, W. Chen, C. W. Thomson, M. S. Ford, S. E. Bosinger, S. Der, Z.-x. Zhang, et al. Expression Profiling of Murine Double-Negative Regulatory T Cells Suggest Mechanisms for Prolonged Cardiac Allograft Survival J. Immunol., April 15, 2005; 174(8): 4535 - 4544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Pardo, M. Buferne, M.-J. Martinez-Lorenzo, J. Naval, A.-M. Schmitt-Verhulst, C. Boyer, and A. Anel Differential implication of protein kinase C isoforms in cytotoxic T lymphocyte degranulation and TCR-induced Fas ligand expression Int. Immunol., December 1, 2003; 15(12): 1441 - 1450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Tremblay, A. M. Wolbink, Y. Cormier, and C. E. Hack Granzyme Activity in the Inflamed Lung Is Not Controlled by Endogenous Serine Proteinase Inhibitors J. Immunol., October 1, 2000; 165(7): 3966 - 3969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Fuller and V. L. Braciale Selective Induction of CD8+ Cytotoxic T Lymphocyte Effector Function by Staphylococcus Enterotoxin B J. Immunol., November 15, 1998; 161(10): 5179 - 5186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. A. Spaeny-Dekking, W. L. Hanna, A. M. Wolbink, P. C. Wever, A. J. Kummer, A. J. G. Swaak, J. M. Middeldorp, H. G. Huisman, C. J. Froelich, and C. E. Hack Extracellular Granzymes A and B in Humans: Detection of Native Species During CTL Responses In Vitro and In Vivo J. Immunol., April 1, 1998; 160(7): 3610 - 3616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Liu, Nussbaum SR, and H. Eisen Hormone conjugated with antibody to CD3 mediates cytotoxic T cell lysis of human melanoma cells Science, January 22, 1988; 239(4838): 395 - 398. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
