The Journal of Immunology, Vol 138, Issue 11 3808-3811, Copyright © 1987 by American Association of Immunologists

ARTICLES

Interleukin 1-driven secretion of interleukin 2 is highly temperature- dependent

HM Lederman, CR Brill and PA Murphy

To investigate the temperature dependence of T lymphocyte activation, we have examined the IL 1-induced secretion of IL 2 by the murine T lymphoma cell line LBRM-33-1A5. During 24-hr incubations, there are modest increases in IL 2 secretion as culture temperatures are increased from 33 degrees to 37 degrees C, but IL 2 secretion declines at higher temperatures. However, the kinetics of IL 2 release during the first 8 hr of culture are highly temperature-dependent. The rate of IL 2 release increases linearly with temperature over the range from 33 degrees to 41 degrees C, demonstrating temperature coefficients (Q10) greater than 70. In contrast, IL 2-promoted proliferation of a continuous T cell line is much less temperature-dependent with Q10 values of less than 4.0.

This article has been cited by other articles:

				P.K. Coyle, L.B. Krupp, C. Doscher, Zhidian Deng, and A. Milazzo Clinical and Immunological Effects of Cooling in Multiple Sclerosis Neurorehabil Neural Repair, January 1, 1996; 10(1): 9 - 15. [Abstract] [PDF]

				P. Menasche, S. Haydar, J. Peynet, C. D. Buit, R. Merval, G. Bloch, A. Piwnica, and A. Tedgui A potential mechanism of vasodilation after warm heart surgery: The temperature-dependent release of cytokines J. Thorac. Cardiovasc. Surg., January 1, 1994; 107(1): 293 - 299. [Abstract] [Full Text]