The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lynch, J. M.
Right arrow Articles by Henson, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lynch, J. M.
Right arrow Articles by Henson, P. M.

The Journal of Immunology, Vol 137, Issue 8 2653-2661, Copyright © 1986 by American Association of Immunologists

ARTICLES

The intracellular retention of newly synthesized platelet-activating factor

JM Lynch and PM Henson

The ether phospholipid platelet-activating factor (PAF) has been generally assumed to be released into the extracellular environment by the cells of origin, whereupon it effects its well-known mediator functions. However, during the generation of PAF by human neutrophils, it was noted that the majority of the measurable PAF remained associated with the cells. Accordingly, the intracellular and extracellular distribution of PAF was examined in neutrophils and several other cell types. No PAF was detected in association with unstimulated neutrophils. However, in stimulated neutrophils, PAF was produced and the majority of this material remained in association with the cells independent of the type of stimulus, dose of stimulus, or method of cell isolation used. This pattern of cell association of PAF was seen in all but one of the cell types tested. The retention of PAF by stimulated neutrophils was not due to spurious underestimates of the extracellular levels due to extracellular metabolism and inactivation of released PAF, nor to release followed by readsorption or binding of PAF to the cells. The retention of PAF also occurred in the presence of plasma and appears to be a common phenomenon. Thus, the majority of newly synthesized PAF appears to be retained within the cell and not released.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. P. O'Dea, M. R. Wilson, J. O. Dokpesi, K. Wakabayashi, L. Tatton, N. van Rooijen, and M. Takata
Mobilization and Margination of Bone Marrow Gr-1high Monocytes during Subclinical Endotoxemia Predisposes the Lungs toward Acute Injury
J. Immunol., January 15, 2009; 182(2): 1155 - 1166.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
H. S. Leong, B. M. Mahesh, J. R. Day, J. D. Smith, A. D. McCormack, G. Ghimire, T. J. Podor, and M. L. Rose
Vimentin autoantibodies induce platelet activation and formation of platelet-leukocyte conjugates via platelet-activating factor
J. Leukoc. Biol., February 1, 2008; 83(2): 263 - 271.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Watanabe, G. K. Marathe, P. O. Neilsen, A. S. Weyrich, K. A. Harrison, R. C. Murphy, G. A. Zimmerman, and T. M. McIntyre
Endotoxins Stimulate Neutrophil Adhesion Followed by Synthesis and Release of Platelet-activating Factor in Microparticles
J. Biol. Chem., August 29, 2003; 278(35): 33161 - 33168.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. M. Marrache, F. Gobeil Jr., S. G. Bernier, J. Stankova, M. Rola-Pleszczynski, S. Choufani, G. Bkaily, A. Bourdeau, M. G. Sirois, A. Vazquez-Tello, et al.
Proinflammatory Gene Induction by Platelet-Activating Factor Mediated Via Its Cognate Nuclear Receptor
J. Immunol., December 1, 2002; 169(11): 6474 - 6481.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
Y.S. Ho, P.J. Sheffield, J. Masuyama, H. Arai, J. Li, J. Aoki, K. Inoue, U. Derewenda, and Z.S. Derewenda
Probing the substrate specificity of the intracellular brain platelet-activating factor acetylhydrolase
Protein Eng. Des. Sel., August 1, 1999; 12(8): 693 - 700.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. Fiedler, P. Wheelan, P. M. Henson, and R. C. Murphy
Exogenous Leukotriene B4 (LTB4) Inhibits Human Neutrophil Generation of LTB4 from Endogenous Arachidonic Acid During Opsonized Zymosan Phagocytosis
J. Pharmacol. Exp. Ther., October 1, 1998; 287(1): 150 - 156.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
B. Bussolati, F. Mariano, A. Cignetti, A. Guarini, V. Cambi, R. Foa, G. Piccoli, and G. Camussi
Platelet-Activating Factor Synthesized by IL-12-Stimulated Polymorphonuclear Neutrophils and NK Cells Mediates Chemotaxis
J. Immunol., August 1, 1998; 161(3): 1493 - 1500.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. M. Miotla, P. K. Jeffery, and P. G. Hellewell
Platelet-activating Factor Plays a Pivotal Role in the Induction of Experimental Lung Injury
Am. J. Respir. Cell Mol. Biol., February 1, 1998; 18(2): 197 - 204.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
P. Wheelan and R. C. Murphy
Metabolism of 6-trans-Isomers of Leukotriene B(4) in Cultured Hepatoma Cells and in Human Polymorphonuclear Leukocytes
J. Biol. Chem., August 25, 1995; 270(34): 19845 - 19852.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1986 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1986 by The American Association of Immunologists, Inc. All rights reserved.