The Journal of Immunology, Vol 135, Issue 3 1564-1572, Copyright © 1985 by American Association of Immunologists

ARTICLES

Defining cytolytic T lymphocyte recognition of chemically modified self. I. Response to trinitrophenyl-H-2Kk

K Handa and S Herrmann

We used purified class I antigen incorporated into liposomes to examine the response of secondary cytolytic T lymphocytes (CTL) to chemically modified self. By generating the secondary response in the presence of T cell helper factor, the level of CTL response was limited by CTL recognition of added antigen rather than by helper cell generation of lymphokines. We found a strong secondary response against chemically modified self when spleen cells from trinitrophenyl (TNP)-primed C3H/HeJ mice were stimulated with a) TNP-modified liposomes containing H-2Kk, b) liposomes containing H-2Kk purified from TNP-modified RDM-4 (H-2k) cells, or c) liposomes containing the limited trypsin proteolysis product of H-2Kk that had been directly modified with TNP. In contrast, we were not able to generate a significant CTL response with unmodified H-2Kk incorporated into vesicles along with TNP- modified membrane components lacking H-2Kk. These results suggest that TNP-modified H-2Kk is a major antigenic site recognized by CTL from C3H/HeJ mice after priming against TNP-modified self.

This article has been cited by other articles:

				C. A. West, C. He, M. Su, J. Rawn, S. Swanson, J. B. Hay, and S. J. Mentzer Stochastic Regulation of Cell Migration from the Efferent Lymph to Oxazolone-Stimulated Skin J. Immunol., February 1, 2001; 166(3): 1517 - 1523. [Abstract] [Full Text] [PDF]