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The Journal of Immunology, 1971, 107: 554-562.
Copyright © 1971 by The American Association of Immunologists, Inc.
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Immunochemistry of the Cell-Wall Carbohydrate of Group L Hemolytic Streptococci1

W. W. Karakawa2, J. E. Wagner and J. H. Pazur

From the Department of Microbiology and the Department of Biochemistry, The Pennsylvania State University, University Park, Pennsylvania

Abstract

A cell-wall carbohydrate consisting of N-acetylglucosamine, N-acetylgalactosamine, galactose and rhamnose was isolated from group L hemolytic streptococci. The antigenic determinant of this carbohydrate consisted of terminal beta-N-acetylglucosamine residues attached to rhamnose side chains of the polymer. Group L carbohydrate treated with an induced enzyme preparation from a soil bacterium no longer gave a precipitin reaction with its homologous antiserum but did so strongly with antigroup G streptococcal serum. The native carbohydrate also gave a precipitin reaction with antisera from rabbits immunized with group A streptococci. The antigenic cross-reactivity between group A and group L carbohydrates was due to the fact that the antigenic determinants of both carbohydrates were terminal beta-N-acetylglucosamine residues. The N-acetylglucosamine residues of group L carbohydrate were extremely acid labile, whereas those of the group A carbohydrate were considerably more stable. This difference indicated some subtle structure differences between group A and group L antigens.

Footnotes

1 This work was supported in part by Research Grants AI-08015 and AM-10822 from the National Institutes of Health and by Grant GB-7220 from the National Science Foundation.

2 Present address: Department of Research Medicine, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104.






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