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The Journal of Immunology, 1970, 105: 416-425.
Copyright © 1970 by The American Association of Immunologists, Inc.
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Antigenic Analysis of Human Heart Tissue. Antigens with Restricted Organ Distribution in Acid Extracts of Human Myocardium1,2,

Enrique Espinosa and Melvin H. Kaplan3

From the Department of Medicine, Metropolitan General Hospital, Case Western Reserve University School of Medicine, Cleveland, Ohio

Abstract

Antigens of human myocardium solbuilized by acid extraction at pH 2.5 were submitted to further analysis by double diffusion and immunoelectrophoresis using rabbit antisera to fractions of human heart acid extract obtained by DEAE-cellulose chromatography. Combined immunodiffusion and specific absorption tests demonstrated at least six antigens common to cardiac and skeletal muscle in acid extracts of myocardium. An additional heart-specific antigen was demonstrated in such acid extracts as well as in saline washes of myocardium. Of the antigens common to cardiac and skeletal muscle, four were found present in the different mammalian species tested, and two showed specificity for primates. The heart-specific antigen exhibited both species-specific and species-cross-reactive antigenic properties. Rabbit antisera to human heart fractions were reactive also with rabbit antigen common to heart and skeletal muscle and with rabbit heart-specific antigen. The antigens common to cardiac and skeletal muscle varied in susceptibility to proteolytic digestion by Pronase, trypsin and pepsin. The heart-specific antigen was susceptible to all three enzymes.

Footnotes

1 Presented in part at the 52nd Annual Meeting of the Federation of American Societies for Experimental Biology, April 1968, Atlantic City, New Jersey.

2 Supported by Grant H-3726 from the National Heart Institute, United States Public Health Service.

3 Research Career Awardee, United States Public Health Service (K-HE-4576).






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