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The in vitro Release of Leukocyte Histamine by Staphylococcal Antigens

From the Departments of Medicine, Medical College of Georgia, and Indiana University School of Medicine, Indianapolis, Indiana

Abstract

Leukocyte histamine was released in vitro by crude staphylococcal antigen supernatants containing protein antigen A or by electrophoretically separated protein antigen A. Leukocyte histamine was not released by similar antigen supernatants from a strain which did not contain protein antigen A, nor by teichoic acids or teichoic acid-mucopeptide complexes. Pre-incubation of antigens with sera containing antigen A precipitins consistently blocked leukocyte histamine release.