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Differential Effect of Heterologous Anti-Lymphocyte Serum on Antibody-Producing Cells and Antigen-Sensitive Cells

From the Department of Bacteriology, Karolinska Institutet, Stockholm 60, and Laboratory of Transplantation Immunology, and Department of Surgery Serafimerlasarettet, Stockholm K, Sweden

Abstract

Rabbit antiserum to mouse lymphocytes (anti-lymphocyte serum (ALS)) did not suppress the primary cellular 19 S or 7 S immune response to sheep red blood cells when given after the antigen. Furthermore, ALS failed to decrease the number of antibody-producing cells when injected into animals already hyperimmunized against sheep red blood cells, but had marked suppressing effect on the antigen-sensitive cells in these animals. This was investigated by a transfer system: hyperimmune spleen cells from ALS-treated and untreated donors, respectively, were mixed with sheep red blood cells and inoculated into irradiated (600 r) syngeneic, nonimmunized recipients. The number of 19 S and 7 S antibody-producing spleen cells was determined 7 days later. Transfer of spleen cells from ALS-untreated donors resulted in excessive cellular 7 S production, in the recipients. The total number of 7 S cells exceeded 10⁶ per spleen and the proportion varied between 1 and 50% as a rule. Pretreatment of the immunized donors by ALS caused a reduction of more than 90% of the number and proportion of 7 S antibody-producing cells in the secondary hosts. Analogous results were obtained for 19 S production. The effect could not be ascribed to destruction of ALS-treated cells in the secondary recipients. The results suggest a differential effect of ALS on antibody-producing cells and antigen-sensitive cells.

Footnotes

¹ Present address: Veterans Administration Hospital, 1310 24th Avenue South, Nashville, Tenn. 37203.