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The Reactivity of Long-Lived Lymphocytes to Typhoid Vaccine in situ in Rat Popliteal Lymph Nodes¹

From the Biological and Medical Sciences Division, United States Naval Radiological Defense Laboratory, San Francisco, California 94135

Abstract

The cells of the popliteal lymph nodes of rats were labeled with tritiated thymidine for 4 days after primary or secondary injections of OT or after a secondary injection of typhoid vaccine. Thiry-one days after the last dose of tymidine, rats of each group were challenged with typhoid vaccine. Increased numbers of heavily-labeled large lymphocytes and mitotic figures were found in the popliteal nodes for 2 days after challenge with typhoid vaccine regardless of the antigen given prior to labeling. This in situ reaction to typhoid vaccine was demonstrated as long as 7 months after labeling. Reasons indicating that these large lymphoid cells were derived from labeled, long-lived small lymphocytes are discussed. We believe these results confirm the validity of the method of following tritiated thymidine-labeled lymphocytes for long periods of time.

Footnotes

This study was supported by the Bureau of Medicine and Surgery, United States Navy Department. Opinions and assertions herein are those of the authors and are not to be construed as necessarily reflecting the official opinions of the Naval establishment.

² Present address: Department of Pediatrics, Stanford University School of Medicine, Palo Alto, California, 94304.