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Protein a from Staphylococcus Aureus

V. Reaction with Guinea Pig -Globulins¹

From the Department of Microbiology, University of Umeå, Umeå, Sweden

Abstract

By gel diffusion and immunoelectrophoresis protein A from S. aureus was shown to give one precipitation line against normal guinea pig serum and two lines against guinea pig anti-EA serum. The single line of normal serum corresponded to ₂-globulin and the double lines of immune serum to ₁- and ₂-globulin. The two nonidentical lines for ₁- and ₂-globulin could not be explained by heterogeneity of the protein A preparation. No reaction was detected between protein A and guinea pig M-globulin.

Footnotes

This work was supported by grants from the Medical Faculty, University of Umeå, Umeå, Sweden, from the Swedish Cancer Society (Project No. 66:60 and 56-B67-02X) and the Swedish Medical Research Council (Project No. K67-13X-620-03A). Presented in part at Cold Spring Harbor Symposium XXXII, June 1 to 7, 1967, and at Nobel Symposium III, Stockholm, June 12 to 17, 1967.

² Present address: The Wallenberg Laboratory, University of Uppsala, Uppsala, Sweden.