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Communications

Immune Response of Strain 13 Guinea Pigs to Lysine-Containing Polymers of Amino Acids¹

From the Department of Biochemistry, Jefferson Medical College, Philadelphia, Pennsylvania

Abstract

Although it has been reported previously that strain 13 guinea pigs cannot react immunogenically against lysine-containing polymers and polypeptidyl proteins (1–5), reinvestigation of this problem has indicated a major disagreement with these results.

Guinea pigs of inbred strain 13 weighing 300 to 400 g were obtained from the closed colony at the National Institutes of Health, Bethesda, Maryland, and from the Rocky Mountain Laboratory, Missoula, Montana. They were immunized with random polymers of -L-amino acids incorporated in complete Freund's adjuvant. Additional studies employed the polymers of -D-amino acids aggregated with methylated bovine serum albumin (MBSA). The basic protocol was the same as that employed previously (6, 7). Animals were injected twice, at intervals of 2 weeks, with 100 µg of the polymer plus adjuvant. Three weeks after the second injection the animals were bled from the retroorbital sinus and were skintested with 100 µg of the specific polymer.

Footnotes

Supported by Grants A1 07825 and T1 A1 334 from the National Institute of Allergy and Infectious Diseases. Paper 28 in series on Antigenicity of Polypeptides (Poly--Amino Acids).

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