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Antigenic Analysis of Human Heart Tissue

Identification of Antigens with Specificity Restricted to Heart and Skeletal Muscle in Acid Extracts of Myocardium¹

From the Department of Medicine, Metropolitan General Hospital, Case-Western Reserve University School of Medicine, Cleveland, Ohio

Abstract

An acid extraction procedure for the solubilization of cardiac antigens present in washed homogenates of myocardium is described. The organ specificity of antigenic components of acid extracts of human heart was studied by immunodiffusion and immunofluorescent techniques. Two antigens, restricted in organ distribution to heart and skeletal muscle, were demonstrated. As indicated by immunofluorescence, these antigens were both localized to sarcolemmal and subsarcolemmal structures of myocardial fibers. One of these antigens was found widely distributed among mammalian species while the other was found only in primate species. Both antigens were susceptible to digestion by trypsin and pepsin, and were found relatively thermolabile. Partial purification of these antigens was obtained by DEAE-cellulose chromatography and by Sephadex G 200 gel filtration. The relationship of heart antigens in such acid extracts to streptococcal cross-reactive antigens(s) is considered.

Footnotes

Supported by Grant H-3726 from the National Heart Institute, United States Public Health Service.

² Research Career Awardee of the United States Public Health Service (K-HE-4576).