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The Journal of Immunology, 1968, 100: 788-792.
Copyright © 1968 by The American Association of Immunologists, Inc.
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Synthesis of the First Component of Guinea Pig Complement by Columnar Epithelial Cells of the Small Intestine

Harvey R. Colten, James M. Gordon, Herbert J. Rapp and Tibor Borsos

From the Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Abstract

The hemolytic plaque assay has been adapted to the study of synthesis of C'1 by individual cells. With this method we found that in the guinea pig the only cells capable of producing plaques due to hemolytic C'1 were the columnar epithelial cells of the small intestine. The production of hemolytic plaques was inhibited by puromycin. None of the other cell types studied were capable of producing hemolytic plaques.






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