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Assignment of Direct and Facilitated Hemolytic Plaques in Mice to Specific Immunoglobulin Classes

From the National Institute of Arthritis and Metabolic Diseases and the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Abstract

The kinetics of appearance of hemolytic plaque-forming cells in immunized mouse spleens has been studied with the aid of immunoglobulin specific antisera and myeloma protein inhibitors. "Facilitated" or "indirect" plaques were developed with antisera specific for 1, 2 and M, and for light chains. Facilitation with anti-M antiserum suggests the presence of two classes of mouse macroglobulin, one responsible for direct plaques and the other requiring an anti-globulin reagent in order to produce lysis. The previously held concept that all indirect plaques represent "7 S" antibody is no longer tenable.

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