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A Demonstration of the Coprecipitation of -Lipoproteins with Specific Precipitates of Chicken Antibodies and Human Serum Albumin

From the Department of Microbiology, Yale University School of Medicine, New Haven, Connecticut

Abstract

The coprecipitation of -lipoprotein with specific precipitates of HSA and chicken antibodies (8% NaCl) has been demonstrated by gas chromatography. The precipitin curves obtained with the -lipoprotein-depleted sera in the presence of EDTA were reproducible despite aging at refrigerator or freezer temperature. The curves for the depleted sera had the same macroglobulin-type contour as the untreated serum, but had lower values throughout the antigen range tested. -Lipoprotein depletion produced no change in equivalence point, but it appeared to decrease the sensitivity of the antibody to 2-ME.

Footnotes

Postdoctoral Trainee supported from United States Public Health Service, National Institute of Allergy and Infectious Diseases Training Grant TI AI 291-02 and from a National Institute of Child Health and Human Development fellowship 5 F 2 HD-22, 715.

² Present address: Department of Obstetrics and Gynecology, University of Iowa, Iowa City, Iowa 52240.