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The Effect of Salt-Induced Aggregation on the Gel Filtration of Chicken 7 S Antibodies

From the Department of Microbiology, Yale University School of Medicine, New Haven, Connecticut

Abstract

A chicken antiserum obtained on the 8th day after immunization has been shown to have the immunoelectrophoretic characteristics of G and a sedimentation coefficient of 6.8 S in 0.35 M formic acid. In 0.87% NaCl, the antibody was eluted from a Biogel P 200 gel filtration column as a protein of approximately 150,000 MW; in contrast, in 5.8% NaCl, the antibody was aggregated, and the bulk of antibody activity was excluded from the gel. This aggregation was confirmed by quantitative immunodiffusion in agar containing 8% NaCl: a D_20,w = 2.3 x 10^-7 cm²/sec was obtained for the antibody. From this diffusion coefficient and the value of 13 S reported for the sedimentation coefficient in 8% NaCl, a MW of 540,000 is calculated for the aggregated antibody.

Footnotes

Postdoctoral Trainee supported from United States Public Health Service National Institute of Allergy and Infectious Diseases Training Grant T1 AI 291-02 and from a National Institute of Child Health and Human Development Fellowship 5 F 2 HD-22, 715.

² Present address: Dept. of Obstetrics and Gynecology, University of Iowa, Iowa City, Iowa 52240.